The Kruger National Park (KNP) said two lions and 110 white- back vultures, and two black ones were killed after they scavenged on a poisoned elephant.
The authority said the sad discovery was made on Saturday in the park.
The elephant carcass was found laced with poison, with gunshots to its head and with its tusks having been removed.
Scavengers in the vicinity of the elephant carcass had been poisoned from feeding on the exposed areas of the carcass.
“It seems poachers have resorted to wildlife poisoning in the National Parks and other protected areas in Southern Africa and we are devastated by these latest mortalities of our wildlife,” said the Managing Executive of KNP, Glenn Phillips.
“Wildlife poisoning is not only a threat to our biodiversity assets, but a single incident can affect hundreds of species – thus cutting their life short and diminishing their ecological role.”
Last year, the Park experienced a similar incident in which one elephant and four African lions suffered the same fate, with 46 vultures and one adult bateleur all dying from poisoning.
An incident of deliberate poisoning of black-backed jackal and other small predators in Addo Elephant National Park in 2014 resembled the same pattern of an attempt to kill wildlife by means of poisoning.
“We would like to thank our anti-poaching teams for their quick response as the outcome could have been worse, had they detected the situation late,” said Phillips.
“Although poisoning has occurred at a low level in the country in recent history, it is a real problem in our neighbouring countries such as Mozambique and Zimbabwe.
“A joint operation with all relevant government security structures, as well as our neighbouring counterparts (in particular those bordering the far northern part of the Park wherein there is a spate of elephant poaching) requires tough regulatory measures to prevent wildlife poisoning and poaching,” concluded Phillips.